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Osmometer Advanced® Model 3250 Single-Sample

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Advanced Instruments Osmometer, Model 3250, for Osmolality Measurement

The Advanced Instruments Model 3250 Single-Sample Osmometer determines the osmolality of solutions using freezing point depression (FPD). Ideal for serum, urine or other bodily fluids in clinical applications; and media or formulations in pharmaceutical, biotech or industrial applications.

Automated, single sample, designed to process a 200-250 μL sample, and two-minute test time. The Model 3250 provides the best accuracy and precision in the market today.

  • 0 to 4000 mOsm/kg H2O measurement range offers ideal versatility
  • 200-250 μL provides the greatest precision in a variety of applications.
  • Multi-language operation
  • Unparalleled ease of use
  • Internal diagnostics
  • On-board statistical analysis
  • Results recall up to the last 30 tests
  • Automated calibration
  • On-board printer
  • Hot-Line® Technical Service available 24/7

Advanced Instruments also supplies ControLine Products

  • ClinitrolTM 290 mOsmol Reference Solution<
  • Protinol® Protein-based Controls
  • RenolTM Urine Osmolality Contols
  • Linearity Osmolality Controls

Method – Freezing Point Depression Osmometry
Osmometry measures the total molar concentration of dissolved solids in any solution. The freezing point of a solution is depressed in direct relation to the amount of solute in solution. Click here for a more detailed explanation.

Uses and Applications – Emergency Medicine, Internal Medicine/Endocrinology, Urology/Renal Function, Surgery/Critical Care, Obstetrics/Gynecology, Pharmacy, General Quality Control. Click here for a more detailed explanation.

Advanced® Model 3250 Osmometer Technical Specifications


Power Requirement: 100 to 250 VAC (50/60 Hz)

Fuses (2): 250V time delay (Type T): 2-Amp (100-130 VAC); 1-Amp (200-250 VAC)

Power Consumption: 95 Watts

Memory Backup: Integral lithium cell; 10-year life (typical); (not user-replaceable)

Sample Volume: 200 to 250µL

Sample Capacity: Single sample

Readout: 20-character vacuum fluorescent display

Units: mOsm/kg H2O

Low: 0 to 2000 mOsm/kg H2O
High: 1400 to 4000 mOsm/kg H2O

Resolution: 1 mOsm/kg H2O

Communications: On-board printer, DTE EIA-232/V.24 (RS-232) serial port and optional barcode scanner

Performance at Reference Conditions

Linearity: Less than 0.5% from a straight line
Repeatability: 0 to 400 mOsm: ±2 mOsm/kg (1 S.D.)  400 to 4000 mOsm: ±0.5% (1 S.D.)
Drift: Less than 1 mOsm/kg H2O per month

Performance Over Operating Conditions

Temperature Effects: Less than 1 mOsm/kg H2O for every 5°C (9°F) ambient temperature change
Operating Conditions Temperature: 18°C to 35°C (64°F to 95°F)
Humidity: 5 to 80% relative humidity (non-condensing)
Storage Temperature: -40°C to +45°C (-40°F to +113°F)
Start-up Time: Immediately from stand-by; under 5 minutes from power-on
Test Time: Approximately 2 minutes per sample in low range; approximately 3 minutes in high range

30.5 cm x 33.0 cm x 45.7 cm
(12.0" H x 13.0" W x 18.0" D)
Height With Head Up:  40.6 cm (16.0" H)

Net:   10.4 kg (23.0 lbs.)
Shipping:   15.4 kg (34.0 lbs.)

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